CellLine
GrowthProperty = 'suspension'
Name = 'J.CaM1.6 (derivative mutant of Jurkat)'
DNA = 'Amelogenin: X,Y \nCSF1PO: 11 \nD13S317: 8,12 \nD16S539: 10,11 \nD5S818: 9 \nD7S820: 8,10 \nTHO1: 6,9.3 \nTPOX: 8,10 \nvWA: 18,19'
Biosafety = '1'
Gender = 'Male'
GrowthMedium = 'The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.'
Disease = 'acute T cell leukemia '
Preservation = 'Freeze medium: Complete growth medium, 95%; DMSO, 5% \nStorage temperature: liquid nitrogen vapor phase'
Applications = 'transfection host (Roche Transfection Reagents)'
Depositors = ' A Weiss'
Comments = 'The J.CaM1.6 cell line is a derivative mutant of Jurkat. \nThe Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. [50685] [112530] \nThe J.CaM1.6 line was derived from the E6-1 clone of Jurkat (ATCC TIB-152) by treatment with ethylmethanesulfonate (EMS). \nCells were then selected for T cell antigen receptor (TCR) expression and inability to increase intracellular calcium in response to TCR stimulation with OKT3 antibody. \nThe T cell antigen receptor appears to be structurally normal (normal \nCD3/T1 complexes are present); however, TCR signal transduction is defective. \nThey are deficient in lck kinase activity and are missing exon 7 in their lck mRNA.'
Shipment = 'frozen'
Morphology = 'lymphoblast'
CatalogueNo = 'CRL-2063'
Organism = 'Homo Sapiens'
Authority = 'ATCC'
Publications = '22635: Straus DB, Weiss A. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell 70: 585-593, 1992. PubMed: 1505025\n23300: Goldsmith MA, Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. USA 84: 6879-6883, 1987. PubMed: 3309950\n27051: Lund T, et al. Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells. J. Virol. 71: 378-382, 1997. PubMed: 8985360\n32563: Cenciarelli C, et al. T cell antigen receptor ubiquitination is a consequence of receptor-mediated tyrosine kinase activation. J. Biol. Chem. 271: 8709-8713, 1996. PubMed: 8621503\n33013: Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141\n33089: Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792\n50685: Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013\n112530: Ronald Wange, personal communication'